Journal: Journal of Cellular and Molecular Medicine

Article Title: Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury

doi: 10.1111/jcmm.12638

Figure Lengend Snippet: Protective effects of BM-MSCs on HPMVECs against oxygen-glucose deprivation (OGD). Phase contrast images of cultured HPMVECs with or without BM-MSCs: (A) Control group; (B) Control+BM-MSCs group; (C) OGD group; (D) OGD+ BM-MSCs group; (E) OGD+FIB group. (F) Administration of BM-MSCs alleviated OGD-induced high permeability in HPMVECs. This experiment was repeated three times in each group. Data are expressed as the mean ± SD. * P < 0.05 versus control group; # P < 0.05 versus OGD group by anova (Bonferroni). FIB: fibroblast.

Article Snippet: Human BM-MSCs and human pulmonary micro-vascular endothelial cells (HPMVECs) were purchased from ScienCell (Carlsbad, CA, USA).

Techniques: Cell Culture, Permeability

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury

doi: 10.1111/jcmm.12638

Figure Lengend Snippet: Effects of in vitro co-culture of OGD-treated HPMVECs with BM-MSCs on the mitochondrial membrane potential (ΔΨm). Red fluorescence indicates that JC-1 aggregates formed in cells with a high ΔΨm, whereas green fluorescence indicates that JC-1 monomers formed in cells with low ΔΨm. (A) Control group; (B) Control+BM-MSCs group; (C) OGD group; (D) OGD+ BM-MSCs group; (E) OGD+FIB group. FIB: fibroblast.

Article Snippet: Human BM-MSCs and human pulmonary micro-vascular endothelial cells (HPMVECs) were purchased from ScienCell (Carlsbad, CA, USA).

Techniques: In Vitro, Co-Culture Assay, Fluorescence

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury

doi: 10.1111/jcmm.12638

Figure Lengend Snippet: Effect of BM-MSCs on apoptosis and necrosis in OGD-induced HPMVECs. Cells were double-stained with Annexin V-FITC and PI and were then analysed using flow cytometry. This experiment was repeated independently three times in each group, and the representative results are shown. (A) Control group; (B) Control+BM-MSCs group; (C) OGD group; (D) OGD+BM-MSCs group; (E) OGD+FIB group. (F) Statistical analysis of the proportions of early apoptosis and later apoptosis of HPMVECs. These results are shown as the mean ± SD. * P < 0.05 versus control group; # P < 0.05 versus OGD group by anova (Bonferroni). FIB: fibroblast.

Article Snippet: Human BM-MSCs and human pulmonary micro-vascular endothelial cells (HPMVECs) were purchased from ScienCell (Carlsbad, CA, USA).

Techniques: Staining, Flow Cytometry

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury

doi: 10.1111/jcmm.12638

Figure Lengend Snippet: The autophagic levels in HPMVECs with or without BM-MSCs. Representative transmission electron microscopy (TEM) images for autophagy ultrastructures and quantitative analysis of the number of autophagosomes in different groups. (A) Control group; (B) Control+BM-MSCs group; (C) OGD group; (D) OGD+BM-MSCs group; (E) OGD+FIB group. (F) Statistical analysis of the number of autophagosomes per μm 2 . Autophagosomes are indicated by arrows. N = cell nucleus. The results are shown as the mean ± SD. * P < 0.05 versus control group; # P < 0.05 versus OGD group by anova (Bonferroni). FIB: fibroblast.

Article Snippet: Human BM-MSCs and human pulmonary micro-vascular endothelial cells (HPMVECs) were purchased from ScienCell (Carlsbad, CA, USA).

Techniques: Transmission Assay, Electron Microscopy

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury

doi: 10.1111/jcmm.12638

Figure Lengend Snippet: BM-MSCs co-culture increased the level of autophagosomes in OGD-induced HPMVECs. Green puncta revealed MDC-labelled autophagosomes. (A) Control group; (B) Control+BM-MSCs group; (C) OGD group; (D) OGD+ BM-MSCs group; (E) OGD+FIB group. FIB: fibroblast.

Article Snippet: Human BM-MSCs and human pulmonary micro-vascular endothelial cells (HPMVECs) were purchased from ScienCell (Carlsbad, CA, USA).

Techniques: Co-Culture Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury

doi: 10.1111/jcmm.12638

Figure Lengend Snippet: BM-MSC co-cultures increased the autophagy level in OGD-induced HPMVECs. The protein levels of LC3, p62, and β-actin were examined using Western blotting analyses. (A) Representative Western image of LC3-I, LC3-II, p62, and p-Akt in OGD-induced HPMVECs with or without BM-MSCs. (B–D) Statistical analysis of the expression of LC3-II/LC3-I, p62/β-actin, and p-Akt/Akt in OGD-induced HPMVECs. The results are shown as the mean ± SD. * P < 0.05 versus control group; # P < 0.05 versus OGD group by anova (Bonferroni). FIB: fibroblast.

Article Snippet: Human BM-MSCs and human pulmonary micro-vascular endothelial cells (HPMVECs) were purchased from ScienCell (Carlsbad, CA, USA).

Techniques: Western Blot, Expressing

Journal: Stem Cell Research & Therapy

Article Title: Low concentration flufenamic acid enhances osteogenic differentiation of mesenchymal stem cells and suppresses bone loss by inhibition of the NF-κB signaling pathway

doi: 10.1186/s13287-019-1321-y

Figure Lengend Snippet: FFA at 50 μM promoted osteogenic differentiation of hBMMSCs in vivo. a H&E staining of PM, PM+DMSO, and PM+FFA groups. b Masson’s staining of PM, PM+DMSO, and PM+FFA groups. PM, proliferation media; FFA, flufenamic acid; hBMMSC, human bone marrow-derived mesenchymal stem cell; H&E, hematoxylin and eosin

Article Snippet: Primary human BMMSCs and human adipose-derived mesenchymal stem cells (hASCs) were purchased from ScienCell Company (San Diego, CA, USA).

Techniques: In Vivo, Staining, Derivative Assay

Journal: Stem Cell Research & Therapy

Article Title: Low concentration flufenamic acid enhances osteogenic differentiation of mesenchymal stem cells and suppresses bone loss by inhibition of the NF-κB signaling pathway

doi: 10.1186/s13287-019-1321-y

Figure Lengend Snippet: FFA in low concentrations promoted the osteogenic differentiation of hMSCs by inhibiting the NF-κB pathway. a FFA at 50 μM downregulated the expression of TNF , IL6 , IL8 , and ICAM1 both in PM and OM in hBMMSCs, as determined by qRT-PCR. b FFA at 50 μM could not increase ALP activity in hBMMSCs in the presence of TNF-α or LPS. Yet FFA at 50 μM increased ALP activity more in hBMMSCs in the presence of Bay117082. Human BMMSCs were treated with PM, OM, OM with FFA at 50 μM, or OM with FFA at 50 μM and TNF-α or LPS or Bay117082 for 7 days before ALP staining. c FFA at 50 μM no longer accelerated mineralization in hBMMSCs in the presence of TNF-α or LPS. Yet FFA at 50 μM accelerated mineralization more in hBMMSCs in the presence of Bay117082. Cells were treated with PM, OM, OM with FFA at 50 μM, or OM with FFA at 50 μM and TNF-α or LPS or Bay117082 for 14 days, and calcium deposition was then tested using ARS staining. d The result of quantification of ALP activity was consistent with the result of ALP staining. e The result of quantification of ARS was consistent with the result of ARS staining. f TNF-α and LPS downregulated the expression of RUNX2 and BGLAP in hBMMSCs, which had been increased by 50 μM FFA, as determined by qRT-PCR. Bay117082 further upregulated the expression of RUNX2 and BGLAP in hBMMSCs, which had been increased by 50 μM FFA, as determined by qRT-PCR. g Western blot of protein expression of p-IKK, p-IκBα, IκBα, p-P65, P65, and the internal control GAPDH. Human BMMSCs were cultured in PM or PM with FFA for 7 days before treated with TNF-α or LPS for 30 min. h Western blot of protein expression of p-IKK, p-IκBα, IκBα, p-P65, P65, RUNX2, and the internal control GAPDH. Human BMMSCs were cultured in OM or OM with FFA for 7 days before treated with TNF-α or LPS for 30 min. All data are shown as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, and *** p < 0.001. FFA, flufenamic acid; hBMMSC, human bone marrow-derived mesenchymal stem cell; PM, proliferation media; OM, osteogenic media; ALP, alkaline phosphatase; ARS, alizarin red S; RUNX2, runt-related transcription factor 2; BGLAP, bone gamma-carboxyglutamate protein; qRT-PCR, quantitative real-time reverse transcription PCR; hMSC, human mesenchymal stem cell; NF-κB, nuclear factor kappa B; TNF-α, tumor necrosis factor alpha

Article Snippet: Primary human BMMSCs and human adipose-derived mesenchymal stem cells (hASCs) were purchased from ScienCell Company (San Diego, CA, USA).

Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Staining, Western Blot, Control, Cell Culture, Derivative Assay, Reverse Transcription

Journal: Immunity, Inflammation and Disease

Article Title: Toxoplasma gondii suppress human cord blood cell differentiation to the NK cell population

doi: 10.1002/iid3.1329

Figure Lengend Snippet: Incubation of human BM‐MSCs with Toxoplasma gondii tachyzoites at different ratios (A) 1:10 and (B) 1:100 for 2 weeks (arrow points to BM‐MSCs). By increasing tachyzoites numbers, BM‐MSCs exhibited cytotoxic indicated with the reduction of cell number per cm 2 and loss of spindle shape morphology. Incubation of human BM‐MSCs with PTG (C) and RH (D) T. gondii tachyzoites for 2 weeks. (E) Control noninfected MSCs. BM‐MSCs, bone marrow mesenchymal stem cells.

Article Snippet: Commensurate with these comments, BM‐MSCs can be used as suitable cell sources for the expansion of T. gondii culturing., Human BM‐MSCs were obtained from the Stem Cell Research Center (Tabriz University of Medical Sciences).

Techniques: Incubation, Control

Journal: Immunity, Inflammation and Disease

Article Title: Toxoplasma gondii suppress human cord blood cell differentiation to the NK cell population

doi: 10.1002/iid3.1329

Figure Lengend Snippet: Measuring protein levels of CD56 (A) and GZMA (B) in UCB‐MNCs after exposure to RH and PTG Toxoplasma gondii tachyzoites. The results are shown as the mean ± SD of three independent experiments. There was a statistically significant difference in MNC and other groups (* p < .05; ** p < .01, *** p < .001, **** p < .0001). GZMA, granzyme A; UCB‐MNCs, umbilical cord blood mononuclear cells.

Article Snippet: Commensurate with these comments, BM‐MSCs can be used as suitable cell sources for the expansion of T. gondii culturing., Human BM‐MSCs were obtained from the Stem Cell Research Center (Tabriz University of Medical Sciences).

Techniques: